Transcriptome, proteome, and expression analyses showed that MaPPO1 exhibits preferential expression in fruit tissue and is highly expressed at respiratory climacteric during fruit ripening. MaPPOs did not cluster with Rosaceae and Solanaceae, indicating distant affinities, and MaPPO6/7/8/9/10 clustered into an individual group. Phylogenetic tree analysis revealed that MaPPO genes were categorized into five groups. Results and discussion: We found that more than two-thirds of the MaPPO genes had one intron, and all contained three conserved structural domains of PPO, except MaPPO4. Transient expression assay in tobacco leaves was used to identify the subcellular localization of selected MaPPOs, and we analyzed the polyphenol oxidase activity using recombinant MaPPOs and transient expression assay. The expression patterns were analyzed based on omics data and verified by qRT-PCR analysis. Methods: In this study, we analyzed the physicochemical properties, gene structure, conserved structural domains, and evolutionary relationship of the PPO gene family of banana. In banana ( Musa acuminata, AAA group), 10 PPO genes were determined based on the availability of a high-quality genome sequence, but the role of PPO genes in fruit browning remains unclear. They can catalyze the oxidization of polyphenols and result in the browning of damaged or cut fruit, which seriously affects fruit quality and compromises the sale of fruit. Introduction: Polyphenol oxidases (PPOs), which are widely present in plants, play an important role in the growth, development, and stress responses. 3College of Life Sciences, South China Agricultural University, Guangzhou, China.2Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China. 1Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization (Ministry of Agriculture and Rural Affairs), Guangdong Provincial Key Laboratory of Tropical and Subtropical Fruit Tree Research, Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, China.Fei Qin 1,2,3 Chunhua Hu 1 Tongxin Dou 1 Ou Sheng 1 Qiaosong Yang 1 Guiming Deng 1 Weidi He 1 Huijun Gao 1 Chunyu Li 1 Tao Dong 1 Ganjun Yi 1,2* Fangcheng Bi 1,2*
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